DYRK1A Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4176
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4176-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
After reconstitution, the DYRK1A antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) human HeLa, 2) human MCF7, 3) rat skeletal muscle and 4) mouse skeletal muscle lysate with DYRK1A antibody at 0.5ug/ml. Predicted molecular weight ~85 kDa, observed here at ~100 kDa.
2 / 3
IF/ICC staining of FFPE human U-2 OS cells with DYRK1A antibody (red) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
3 / 3
Flow cytometry testing of human SiHa cells with DYRK1A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DYRK1A antibody.
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Western blot testing of 1) human HeLa, 2) human MCF7, 3) rat skeletal muscle and 4) mouse skeletal muscle lysate with DYRK1A antibody at 0.5ug/ml. Predicted molecular weight ~85 kDa, observed here at ~100 kDa.
IF/ICC staining of FFPE human U-2 OS cells with DYRK1A antibody (red) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human SiHa cells with DYRK1A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DYRK1A antibody.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunofluorescence/Immunocytochemistry (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the DYRK1A antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Dual specificity tyrosine-phosphorylation-regulated kinase 1A is an enzyme that in humans is encoded by the DYRK1A gene. This gene encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. Alternative splicing of this gene generates several transcript variants differing from each other either in the 5' UTR or in the 3' coding region. These variants encode at least five different isoforms.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids H38-M269 was used as the immunogen for the DYRK1A antibody.
Limitation:
This DYRK1A antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q13627