Dystrophin Antibody

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NSJ Bioreagents
Product Code: NSJ-R31689
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31689-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Dystrophin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) human HEK293, 2) human K562 and 3) mouse HEPA1-6 lysate with Dystrophin antibody at 0.5ug/ml. Predicted molecular weight ~427 kDa.
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IHC-P: Dystrophin antibody testing of human lung cancer tissue
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IHC-P: Dystrophin antibody testing of mouse brain tissue
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IHC-P: Dystrophin antibody testing of rat heart tissue
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Flow cytometry testing of human HepG2 cells with Dystrophin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Dystrophin antibody.
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Western blot testing of 1) human HEK293, 2) human K562 and 3) mouse HEPA1-6 lysate with Dystrophin antibody at 0.5ug/ml. Predicted molecular weight ~427 kDa.
IHC-P: Dystrophin antibody testing of human lung cancer tissue
IHC-P: Dystrophin antibody testing of mouse brain tissue
IHC-P: Dystrophin antibody testing of rat heart tissue
Flow cytometry testing of human HepG2 cells with Dystrophin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Dystrophin antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Dystrophin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Dystrophin, also known as DMD, is a rod-shaped cytoplasmic protein, and a vital part of a protein complex that connects the cytoskeleton of a muscle fiber to the surrounding extracellular matrix through the cell membrane. It is mapped to Xp21.2-p21.1. This complex is variously known as the costamere or thedystrophin-associated protein complex. Many muscle proteins, such as alpha-dystrobrevin, syncoilin, synemin, sarcoglycan, dystroglycan, and sarcospan, colocalize with Dystrophin at the costamere. Dystrophin is a protein located between the sarcolemma and the outermost layer of myofilaments in the muscle fiber (myofiber). It is a cohesive protein, linking actin filaments to another support protein that resides on the inside surface of each muscle fiber?s plasma membrane (sarcolemma).
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
1756
Immunogen:
Human partial recombinant protein (AA 3076-3404) was used as the immunogen for this Dystrophin antibody.
Limitation:
This Dystrophin antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat