EBP1 Antibody / ErbB3-binding protein 1

NSJ Bioreagents
Product Code: NSJ-R32616
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32616-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the EBP1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Western blot testing of 1) rat liver, 2) mouse NIH3T3 and 3) human HepG2 lysate with EBP1 antibody at 0.5ug/ml. Observed molecular weight 42 kDa (p42 isoform) and 48 kDa (p48 isoform).
2 / 4
IHC testing of FFPE mouse intestine tissue with EBP1 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
3 / 4
IHC testing of FFPE mouse brain tissue with EBP1 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
4 / 4
IHC testing of FFPE rat intestine tissue with EBP1 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of 1) rat liver, 2) mouse NIH3T3 and 3) human HepG2 lysate with EBP1 antibody at 0.5ug/ml. Observed molecular weight 42 kDa (p42 isoform) and 48 kDa (p48 isoform).
IHC testing of FFPE mouse intestine tissue with EBP1 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE mouse brain tissue with EBP1 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE rat intestine tissue with EBP1 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the EBP1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
Proliferation-associated protein 2G4 (PA2G4), also known as ErbB3-binding protein 1 (EBP1), is a protein that in humans is encoded by the PA2G4 gene. This gene encodes an RNA-binding protein that is involved in growth regulation. This protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing. This protein can interact with the cytoplasmic domain of the ErbB3 receptor and may contribute to transducing growth regulatory signals. This protein is also a transcriptional corepressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 138-178 (RKADVIKAAHLCAEAALRLVKPGNQNTQVTEAWNKVAHSFN-human) were used as the immunogen for the EBP1 antibody.
Limitation:
This antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9UQ80