EEA1 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4105
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4105-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the EEA1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) HeLa, 2) placenta, 3) COLO320, 4) 22RV1 and 5) A549 lysate with EEA1 antibody at 0.5ug/ml. Predicted molecular weight ~162 kDa.
2 / 2
Western blot testing of rat 1) spleen, 2) thymus, 3) brain, 4) lung and mouse 5) spleen, 6) brain, 7) HEPA1-6 and 8) NIH3T3 lysate with EEA1 antibody at 0.5ug/ml. Predicted molecular weight ~162 kDa.
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Western blot testing of human 1) HeLa, 2) placenta, 3) COLO320, 4) 22RV1 and 5) A549 lysate with EEA1 antibody at 0.5ug/ml. Predicted molecular weight ~162 kDa.
Western blot testing of rat 1) spleen, 2) thymus, 3) brain, 4) lung and mouse 5) spleen, 6) brain, 7) HEPA1-6 and 8) NIH3T3 lysate with EEA1 antibody at 0.5ug/ml. Predicted molecular weight ~162 kDa.

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Further Information

Application Details :
Western Blot: 0.5-1ug/ml
Application Note:
Optimal dilution of the EEA1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
The gene EEA1 encodes for the 1400 amino acid protein, Early Endosome Antigen 1. It localizes exclusively to early endosomes and has an important role in endosomal trafficking. EEA1 binds directly to the phospholipid phosphatidylinositol 3-phosphate through its C-terminal FYVE domain and forms ahomodimer through a coiled coil. Furthermore, EEA1 acts as a tethering molecule that couples vesicle docking with SNAREs such as N-ethylmaleimide sensitive fusion protein, bringing the endosomes physically closer and ultimately resulting in the fusion and delivery of endosomal cargo.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids RENQSLQIKHTQALNRKWAEDNEVQN from the human protein were used as the immunogen for the EEA1 antibody.
Limitation:
This EEA1 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q15075