EIF2S1 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-R31138
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31138-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the EIF2S1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 3
Western blot testing of EIF2S1 antbody; Lane 1: COLO320; 2: CEM; 3: Raji; 4: HT1080 cell lysate. Predicted molecular weight: 36 kDa.
2 / 3
IHC-P: EIF2S1 antibody testing of human intestine cancer tissue
3 / 3
IHC-P: EIF2A antibody testing of human placenta tissue
❮❯

Western blot testing of EIF2S1 antbody; Lane 1: COLO320; 2: CEM; 3: Raji; 4: HT1080 cell lysate. Predicted molecular weight: 36 kDa.
IHC-P: EIF2S1 antibody testing of human intestine cancer tissue
IHC-P: EIF2A antibody testing of human placenta tissue

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the EIF2S1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Eukaryotic Translation Initiation Factor 2, Subunit 1, also called EIF2-alpha or EIF2A, is a protein that in humans is encoded by the EIF2S1 gene. Hartz(2010) mapped the gene to chromosome 14q23.3 based on an alignment of the EIF2S1 sequence with the genomic sequence (GRCh37). Ernst et al.(1987) stated that protein synthesis is inhibited due to phosphorylation of EIF2S1 in hemin-deprived rabbit reticulocyte lysates. HeLa cells subjected to heat shock, serum deprivation, or interferon treatment followed by virus infection also show a correlation between EIF2S1 phosphorylation and translational repression. Jacob et al.(1989) found that the alpha-Pal transcription factor bound to two palindromic sites within the EIF2S1 promoter and was essential for transcription of the gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the middle region of human Eukaryotic Translation Initiation Factor 2 subunit 1 (KDEQLESLFQRTAWV) was used as the immunogen for this EIF2A antibody.
Limitation:
This EIF2S1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P05198