Emerin Antibody

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NSJ Bioreagents
Product Code: NSJ-R32593
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32593-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Emerin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 9
Western blot testing of 1) rat skeletal muscle, 2) mouse heart and 3) human HeLa lysate with Emerin antibody at 0.5ug/ml. Predicted molecular weight 29 kDa but routinely observed at ~34 kDa.
2 / 9
Immunofluorescent staining of FFPE human esophageal squamous cell carcinoma with Emerin antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
3 / 9
Immunofluorescent staining of FFPE human esophageal squamous cell carcinoma with Emerin antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
4 / 9
IF/ICC staining of FFPE human U-2 OS with Emerin antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
5 / 9
IHC staining of FFPE human endometrial carcinoma with Emerin antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
6 / 9
IHC staining of FFPE human esophageal squamous cell carcinoma with Emerin antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
7 / 9
IHC staining of FFPE human colon cancer with Emerin antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
8 / 9
Flow cytometry testing of human U-2 OS cells with Emerin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Emerin antibody.
9 / 9
Immunofluorescent staining of FFPE human lung cancer tissue with Emerin antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: boil tissue sections in pH8 EDTA for 10-20 min and allow to cool before testing.
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Western blot testing of 1) rat skeletal muscle, 2) mouse heart and 3) human HeLa lysate with Emerin antibody at 0.5ug/ml. Predicted molecular weight 29 kDa but routinely observed at ~34 kDa.
Immunofluorescent staining of FFPE human esophageal squamous cell carcinoma with Emerin antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human esophageal squamous cell carcinoma with Emerin antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IF/ICC staining of FFPE human U-2 OS with Emerin antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human endometrial carcinoma with Emerin antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human esophageal squamous cell carcinoma with Emerin antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human colon cancer with Emerin antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Flow cytometry testing of human U-2 OS cells with Emerin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Emerin antibody.
Immunofluorescent staining of FFPE human lung cancer tissue with Emerin antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: boil tissue sections in pH8 EDTA for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Immunofluorescence/Immunocytochemistry (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Emerin antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
Emerin is a serine-rich nuclear membrane protein that in humans is encoded by the EMD gene. And this gene is mapped to Xq28. Emerin is a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Emery?Dreifuss muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the EMD (also known clinically as STA) gene. Emerin appears to be involved in mechanotransduction, as emerin-deficient mouse fibroblasts failed to transduce normal mechanosensitive gene expression responses to strain stimuli. In cardiac muscle, emerin is also found complexed to beta-catenin at adherens junctions of intercalated discs, and cardiomyocytes from hearts lacking emerin showed beta-catenin redistribution as well as perturbed intercalated disc architecture and myocyte shape. This interaction appears to be regulated by glycogen synthase kinase 3 beta.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 1-48 (MDNYADLSDTELTTLLRRYNIPHGPVVGSTRRLYEKKIFEYETQRRRL-human) were used as the immunogen for the Emerin antibody.
Limitation:
This antibody is available for research use only.
Localization:
Nuclear membrane, nuclear
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P50402