EPHA2 Antibody / Eph Receptor A2

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NSJ Bioreagents
Product Code: NSJ-RQ4038
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4038-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
After reconstitution, the Eph Receptor A2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) HeLa, 2) U-87 MG, 3) SHG-44, 4) COLO320, 5) SKOV3, 6) A549 and 7) mouse HEPA1-6 cell lysate with Eph Receptor A2 antibody at 0.5ug/ml. Expected molecular weight: 108~130 kDa.
2 / 3
Flow cytometry testing of human A549 cells with Eph Receptor A2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Eph Receptor A2 antibody.
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Flow cytometry testing of human U-2 OS cells with Eph Receptor A2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Eph Receptor A2 antibody.
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Western blot testing of human 1) HeLa, 2) U-87 MG, 3) SHG-44, 4) COLO320, 5) SKOV3, 6) A549 and 7) mouse HEPA1-6 cell lysate with Eph Receptor A2 antibody at 0.5ug/ml. Expected molecular weight: 108~130 kDa.
Flow cytometry testing of human A549 cells with Eph Receptor A2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Eph Receptor A2 antibody.
Flow cytometry testing of human U-2 OS cells with Eph Receptor A2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Eph Receptor A2 antibody.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Eph receptor A2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
EPHA2 (Ephrin type-A receptor 2) also known as ECK, is a protein that in humans is encoded by the EPHA2 gene. This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. By somatic cell hybrid analysis and fluorescence in situ hybridization, the EPHA2 gene is mapped to chromosome 1p36.1. EPHA2 was readily detectable in human lens fiber cells using immunoblot and immunohistochemistry. EGFR and EPHA2 mediated HCV entry by regulating CD81 -claudin-1 (CLDN1) coreceptor associations and viral glycoprotein-dependent membrane fusion.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human partial protein corresponding to amino acids M851-N970 was used as the immunogen for the Eph Receptor A2 antibody.
Limitation:
This Eph Receptor A2 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P29317