ERBB4 Antibody (HER4)

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NSJ Bioreagents
Product Code: NSJ-R30981
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30981-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the ERBB4 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of ERBB4 antibody and Lane 1: human HeLa; 2: (h) U87; 3: mouse Neuro-2a cell lysate. Predicted molecular weight: 147-180 kDa (precursor), 120, 80 kDa (cleaved forms).
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Western blot testing of ERBB4 antibody and Lane 1: A549; 2: HeLa cell lysate
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Western blot testing of ERBB4 antibody and Lane 1: Recombinant human protein 10ng; 2: 5ng; 3: 2.5ng; 4: 1.25ng
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Western blot testing of ERBB4 antibody and Lane 1: human HeLa; 2: (h) U87; 3: mouse Neuro-2a cell lysate. Predicted molecular weight: 147-180 kDa (precursor), 120, 80 kDa (cleaved forms).
Western blot testing of ERBB4 antibody and Lane 1: A549; 2: HeLa cell lysate
Western blot testing of ERBB4 antibody and Lane 1: Recombinant human protein 10ng; 2: 5ng; 3: 2.5ng; 4: 1.25ng

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the ERBB4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
V-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4, also known as HER4, is an enzyme that in humans is encoded by the ERBB4 gene. The HER4/ERBB4 gene is a member of the type I receptor tyrosine kinase subfamily that includes EGFR, ERBB2 and ERBB3. ERBB4 is a single-pass type I transmembrane protein with multiplefurin-like cysteine rich domains, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and aPDZ domainbinding motif.Huang et al.(2000) reported that the proteinis enriched in the postsynaptic density and associates with PSD95. Itis a transmembrane receptor tyrosine kinase that regulates cell proliferation and differentiation. After binding its ligand, heregulin, or activation of protein kinase C by TPA, the ERBB4 ectodomain is cleaved by a metalloprotease. Ni et al.(2001)concluded that gamma-secretase cleavage ofthe proteinmay represent another mechanism for receptor tyrosine kinase-mediated signaling.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of human ErbB4/HER4 (KLSSLSDLEQQYRALRK) was used as the immunogen for this ERBB4 antibody (100% homologous in human, mouse and rat).
Limitation:
This ERBB4 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q15303