ERK1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30409
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30409-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the ERK1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of ERK1 antibody and Lane 1: rat spleen; 2: rat thymus; 3: rat skeletal muscle; 4: rat kidney; 5: HeLa; 6: Jurkat; 7: Raji; 8: COLO320~
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IHC-P: ERK1 antibody testing of human breast cancer tissue
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Western blot testing of ERK1 antibody and Lane 1: rat spleen; 2: rat thymus; 3: rat skeletal muscle; 4: rat kidney; 5: HeLa; 6: Jurkat; 7: Raji; 8: COLO320~
IHC-P: ERK1 antibody testing of human breast cancer tissue

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the ERK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Mitogen-activated protein kinase 3 is an enzyme that in humans is encoded by the MAPK3 gene.The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases(ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. MAPK3 gene is mapped to human chromosome 16 by hybrid cell panel analysis.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the middle region of human ERK1 (KTKVAWAKLFPKSDS) was used as the immunogen for this ERK1 antibody (100% homologous in human, mouse and rat).
Limitation:
This ERK1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P27361