ERp57 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30764
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30764-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the ERp57 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of ERp57 antibody and Lane 1: SMMC-7721; 2: A549; 3: U87; 4: HeLa; 5: MCF-7 cell lysate~
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IHC-P: ERp57 antibody testing of human placenta tissue
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Western blot testing of ERp57 antibody and Lane 1: SMMC-7721; 2: A549; 3: U87; 4: HeLa; 5: MCF-7 cell lysate~
IHC-P: ERp57 antibody testing of human placenta tissue

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the ERp57 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PDIA3 (Protein disulfide isomerase family A, member 3), also called GRP58, ERp57 or ER60, is an isomerase enzyme, mapped on 15q15.3. The PDIA3 protein has protein disulfide isomerase activity. It is also part of the major histocompatibility complex(MHC) class I peptide-loading complex, which is essential for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface. Koivunen et al. showed that PDIA3/ERp57 could not substitute for the beta subunit of PDI. When coexpressed with alpha prolyl 4-hydroxylase, it did not form prolyl 4-hydroxylase tetramers, nor did it have prolyl 4-hydroxylase activity. Hirano et al. expressed human ERp57 and found that the protein had a thiol-dependent reductase activity. They showed that the expression level increased after oncogenic transformation of normal rat kidney cells and NIH 3T3 cells.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 172-188 (LKAASNLRDNYRFAHTN) were used as the immunogen for this ERp57 antibody (100% homologous in human, mouse and rat).
Limitation:
This ERp57 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P30101