Estrogen Inducible Protein pS2 / TFF1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R32898
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32898-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the pS2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC testing of FFPE mouse stomach tissue with pS2 antibody at 1ug/ml. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC testing of FFPE mouse stomach tissue with pS2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
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Western blot testing of mouse stomach lysate samples with pS2 antibody at 0.5ug/ml. Predicted molecular weight ~12 kDa.
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IHC testing of FFPE mouse stomach tissue with pS2 antibody at 1ug/ml. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC testing of FFPE mouse stomach tissue with pS2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Western blot testing of mouse stomach lysate samples with pS2 antibody at 0.5ug/ml. Predicted molecular weight ~12 kDa.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,ELISA (matched pair-capture): 2-8ug/ml,ELISA (matched pair-detect): 0.1-0.5ug/ml
Application Note:
Optimal dilution of the pS2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Description:
TFF1 (Trefoil factor 1), also known as pS2, is a protein that in humans is encoded by the TFF1 gene. Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. It is found that TFF1 in normal human urine inhibited the growth of calcium oxalate crystals. Urinary TFF1 showed an inhibitory potency similar to that of nephrocalcin, and inhibition was dose dependent and inhibited by TFF1 antisera, particularly by antisera directed to the TFF1 C terminus. Concentrations and relative amounts of TFF1 in the urine of patients with idiopathic calcium oxalate kidney stones were significantly less than those found in controls. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant mouse protein corresponding to amino acids Q22-F87 was used as the immunogen for the Estrogen Inducible Protein pS2 antibody.
Limitation:
This pS2 antibody is available for research use only.
Localization:
Cytoplasmic, secreted
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q08423