FASN Antibody / Fatty acid synthase

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NSJ Bioreagents
Product Code: NSJ-R32114
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32114-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the FASN antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 6
Immunofluorescent staining of FFPE human ovarian cancer tissue with FASN antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
2 / 6
Immunofluorescent staining of FFPE human rectal cancer tissue with FASN antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
3 / 6
Immunofluorescent staining of FFPE human T47-D cells tissue with FASN antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
4 / 6
IHC testing of FFPE human intestinal cancer tissue with FASN antibody. HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to staining.
5 / 6
Western blot testing of human 1) HeLa, 2) HepG2, 3) HCCT, 4) 293T, 5) MCF7, 6) RT4 and 7) A549 cell lysate with FASN antibody. Expected molecular weight ~270 kDa.
6 / 6
Western blot testing of 1) rat liver, 2) mouse lung, 3) mouse liver and 4) mouse NIH 3T3 cell lysate with FASN antibody. Expected molecular weight ~270 kDa.
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Immunofluorescent staining of FFPE human ovarian cancer tissue with FASN antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human rectal cancer tissue with FASN antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human T47-D cells tissue with FASN antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human intestinal cancer tissue with FASN antibody. HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to staining.
Western blot testing of human 1) HeLa, 2) HepG2, 3) HCCT, 4) 293T, 5) MCF7, 6) RT4 and 7) A549 cell lysate with FASN antibody. Expected molecular weight ~270 kDa.
Western blot testing of 1) rat liver, 2) mouse lung, 3) mouse liver and 4) mouse NIH 3T3 cell lysate with FASN antibody. Expected molecular weight ~270 kDa.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,ICC (Paraffin): 0.5-1ug/ml
Application Note:
Optimal dilution of the FASN antibody should be determined by the researcher.
Description:
Fatty acid synthase (FAS) is an enzyme that in humans is encoded by the FASN gene. The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 1-226 of human FASN were used as the immunogen for the FASN antibody.
Limitation:
This FASN antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P49327