FGF2 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30108
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30108-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
After reconstitution, the FGF2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human SiHa cells with FGF2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) HeLa, 2) SK-O-V3, 3) SiHa and 4) U87 MG cell lysate with FGF2 antibody. Predicted molecular weight: 17-31 kDa (multiple isoforms).
3 / 3
Flow cytometry testing of fixed and permeabilized human SiHa cells with FGF2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FGF2 antibody.
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Immunofluorescent staining of FFPE human SiHa cells with FGF2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) SK-O-V3, 3) SiHa and 4) U87 MG cell lysate with FGF2 antibody. Predicted molecular weight: 17-31 kDa (multiple isoforms).
Flow cytometry testing of fixed and permeabilized human SiHa cells with FGF2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FGF2 antibody.

Documents

Further Information

Application Details :
Western blot (recombinant protein): 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,ELISA: 0.1-0.5ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the FGF2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
FGF2 has been implicated in a multitude of physiologic and pathologic processes, including limb development, angiogenesis, wound healing, and tumor growth. Human FGF2 shares 96% and 97% amino acid sequence homology with mouse and rat respectively. It belongs to the fibroblast growth factor (FGF) family. FGFs exhibit widespread mitogenic and neurotrophic activities. Nine members of the family are currently known, and FGF1 and -2 are present in relatively high levels in CNS. FGF2 is expressed at low levels in many tissues and cell types and reaches high concentrations in brain and pituitary.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Human partial recombinant protein (AA 143-288) was used as the immunogen for this FGF2 antibody.
Limitation:
This FGF2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P09038