FHIT Antibody

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NSJ Bioreagents
Product Code: NSJ-R31595
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31595-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the FHIT antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human U-2 OS cells with FHIT antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
Western blot testing of 1) human Raji, 2) rat brain and 3) rat kidney tissue lysate with FHIT antibody. Predicted molecular weight: ~17 kDa.
3 / 4
IHC-P: FHIT antibody testing of human breast cancer tissue. HIER: steam section in pH6 citrate buffer for 20 min.
4 / 4
Flow cytometry testing of human U-2 OS cells with FHIT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FHIT antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with FHIT antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human Raji, 2) rat brain and 3) rat kidney tissue lysate with FHIT antibody. Predicted molecular weight: ~17 kDa.
IHC-P: FHIT antibody testing of human breast cancer tissue. HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human U-2 OS cells with FHIT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FHIT antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the FHIT antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Bis(5'-adenosyl)-triphosphatase, also known as fragile histidine triad protein (FHIT) is an enzyme that in humans is encoded by the FHIT gene. This gene, a member of the histidine triad gene family, encodes a diadenosine P1,P3-bis(5'-adenosyl)-triphosphate adenylohydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3p14.2, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Furthermore, FHIT has been shown to synergize with VHL, another tumor suppressor, in protecting against chemically - induced lung cancer. It also acts as a tumor suppressor of HER2/neu driven breast cancer.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
2272
Immunogen:
Human partial recombinant protein (AA 1-147) was used as the immunogen for this FHIT antibody.
Limitation:
This FHIT antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human