FLI1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31300
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31300-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the FLI1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of FLI1 antibody and Lane 1: Jurkat; 2: Raji cell lysate. Predicted/observed molecular weight: ~51kDa.
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IHC-P: FLI1 antibody testing of human tonsil tissue. HIER: steamed with pH6 citrate buffer.
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IHC-P: FLI1 antibody testing of rat spleen tissue. HIER: steamed with pH6 citrate buffer.
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Western blot testing of FLI1 antibody and Lane 1: Jurkat; 2: Raji cell lysate. Predicted/observed molecular weight: ~51kDa.
IHC-P: FLI1 antibody testing of human tonsil tissue. HIER: steamed with pH6 citrate buffer.
IHC-P: FLI1 antibody testing of rat spleen tissue. HIER: steamed with pH6 citrate buffer.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the FLI1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Friend leukemia integration 1, also known as Transcription factor ERGB, is a protein that in humans is encoded by the human FLI1 gene. The protooncogene encodes a member of the ETS family of winged helix-turn-helix transcription factors. This gene is mapped to 11q24.3. Fli1 is activated through retroviral insertional mutagenesis in 90% of F-MuLV-induced erythroleukemias. The constitutive activation of fli-1 in erythroblasts leads to a dramatic shift in the Epo/Epo-R signal transduction pathway, blocking erythroid differentiation, activating the Ras pathway, and resulting in massive Epo-independent proliferation of erythroblasts. These results suggest that Fli1 overexpression in erythroblasts alters their responsiveness to Epo and triggers abnormal proliferation by switching the signaling event(s) associated with terminal differentiation to proliferation.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of human Friend leukemia integration 1 (QQEWINQPVRVNVKREYDHM) was used as the immunogen for this FLI1 antibody (100% homologous in human, mouse and rat).
Limitation:
This FLI1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q01543