Flotillin 1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31142
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31142-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the Flotillin 1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Flotillin 1 antbody; Lane 1: rat lung; 2: (r) brain; 3: (r) ovary; 4: human SMMC-7721; 5: (h) MFC-7 cell lysate. Expected/observed size ~49KD
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Western blot testing of Flotillin 1 antbody; Lane 1: rat lung; 2: (r) brain; 3: (r) ovary; 4: human SMMC-7721; 5: (h) MFC-7 cell lysate. Expected/observed size ~49KD

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Flotillin 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Flotillin 1 is a protein that in humans is encoded by the FLOT1 gene. The International Radiation Hybrid Mapping Consortium mapped the gene to chromosome 6. Bickel et al.(1997) found that mouse Flot1 behaves as a resident integral membrane protein of caveolae. It consistently copurified with Flot2 and with caveolin-1 in the purification of caveolin-rich membranes. Hazarika et al.(1999) found that stable transfection of Flot1, which they called ESA/flotillin-2, in COS-1 cells induced filopodia formation and changed the epithelial morphology to that of neuronal cells. Santamaria et al.(2005) found that prostate tumor overexpressed gene-1 interacted with Flotillin1 in detergent-insoluble membrane fractions. Flotillin1 colocalized with PTOV1 at the plasma membrane and in the nucleus, and it entered the nucleus concomitant with PTOV1 shortly before initiation of S phase.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the middle region of human FLOT1 (KKAAYDIEVNTRRAQA) was used as the immunogen for this Flotillin 1 antibody.
Limitation:
This Flotillin 1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O75955