Flotillin 2 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31143
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31143-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the Flotillin 2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Flotillin 2 antbody; Lane 1: rat lung; 2: 293T cell lysate
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Western blot testing of Flotillin 2 antbody; Lane 1: rat lung; 2: 293T cell lysate

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Further Information

Application Details :
Western blot: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Flotillin 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Flotillin 2, also known as ESA1 or M17S1, is a protein that in humans is encoded by the FLOT2 gene. Schroeder et al.(1991) isolated a cDNA for an epidermal surface antigen believed to be involved in epidermal cell adhesion. By analysis of a somatic cell hybrid panel and in situ hybridization using the FLOT2 cDNA, the gene was mapped to 17q11-q12 in the region containing the NF1 gene. Bickel et al.(1997) found that mouse Flot2 consistently copurifies with Flot1 and with caveolin-1 in the purification of caveolin-rich membranes. Using a quantitative proteomic analysis of cultured mouse neuronal stem cells, Li et al.(2012) found that palmitoylation and oligomerization of Flotillin2 was abolished in homozygous Dhhc5 mutant neuronal stem cells. The absolute amount of Flotillin2 was not changed in Dhhc5 mutant neurons.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of human FLOT2 (TLTVEQIYQDRDQFA) was used as the immunogen for this Flotillin 2 antibody (100% homologous in human, mouse and rat).
Limitation:
This Flotillin 2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q14254