Flotillin 2 Antibody

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NSJ Bioreagents
Product Code: NSJ-R32227
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32227-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Flotillin 2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human A431 cells with Flotillin 2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 10
IHC testing of FFPE human breast cancer tissue with Flotillin-2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
3 / 10
IHC testing of FFPE mouse intestine with Flotillin-2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
4 / 10
IHC testing of FFPE rat kidney with Flotillin-2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
5 / 10
IHC testing of frozen mouse kidney tissue with Flotillin 2 antibody.
6 / 10
IHC testing of frozen rat kidney tissue with Flotillin 2 antibody.
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IHC testing of frozen rat small intestine tissue with Flotillin 2 antibody.
8 / 10
Western blot testing of 1) rat liver, 2) rat lung, 3) mouse liver, 4) mouse lung, 5) mouse testis, 6) human placenta, 7) A375, 8) HeLa, 9) 22RV1 and 10) mouse NIH3T3 lysate with Flotillin 2 antibody. Predicted molecular weight: ~47 kDa.
9 / 10
Flow cytometry testing of human U-2 OS cells with Flotillin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Flotillin 2 antibody.
10 / 10
Flow cytometry testing of rat RH35 cells with Flotillin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Flotillin 2 antibody.
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Immunofluorescent staining of FFPE human A431 cells with Flotillin 2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human breast cancer tissue with Flotillin-2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE mouse intestine with Flotillin-2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat kidney with Flotillin-2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of frozen mouse kidney tissue with Flotillin 2 antibody.
IHC testing of frozen rat kidney tissue with Flotillin 2 antibody.
IHC testing of frozen rat small intestine tissue with Flotillin 2 antibody.
Western blot testing of 1) rat liver, 2) rat lung, 3) mouse liver, 4) mouse lung, 5) mouse testis, 6) human placenta, 7) A375, 8) HeLa, 9) 22RV1 and 10) mouse NIH3T3 lysate with Flotillin 2 antibody. Predicted molecular weight: ~47 kDa.
Flow cytometry testing of human U-2 OS cells with Flotillin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Flotillin 2 antibody.
Flow cytometry testing of rat RH35 cells with Flotillin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Flotillin 2 antibody.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Flotillin 2 antibody should be determined by the researcher.
Description:
FLOT2 (Flotillin 2), also known as ESA1 or M17S1, may act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function. [UniProt]
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 169-344 of human Flotillin 2 were used as the immunogen for the Flotillin 2 antibody.
Limitation:
This Flotillin 2 antibody is available for research use only.
Localization:
Cytoplasmic, membrane
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q14254