FosB Antibody

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NSJ Bioreagents
Product Code: NSJ-R32134
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32134-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Fos B antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC testing of FFPE human pancreatic cancer tissue with Fos B antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
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IHC testing of FFPE human renal cancer tissue with Fos B antibody. HIER: Boil the paraffin sections in pH8 EDTA for 20 minutes and allow to cool prior to staining.
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IHC testing of FFPE human placental tissue with Fos B antibody. HIER: Boil the paraffin sections in pH8 EDTA for 20 minutes and allow to cool prior to staining.
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IHC testing of FFPE mouse intestinal tissue with Fos B antibody. HIER: Boil the paraffin sections in pH8 EDTA for 20 minutes and allow to cool prior to staining.
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IHC testing of FFPE rat intestinal tissue with Fos B antibody. HIER: Boil the paraffin sections in pH8 EDTA for 20 minutes and allow to cool prior to staining.
6 / 7
Western blot testing of 1) rat thymus and mouse 2) thymus, 3) ovary, 4) spleen, 5) kidney, 6) RAW264.7 and human 7) HL-60 and 8) ThP-1 lysate with Fos B antibody. Expected molecular weight ~36 kDa.
7 / 7
Flow cytometry testing of human SiHa cells with Fos B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Fos B antibody.
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IHC testing of FFPE human pancreatic cancer tissue with Fos B antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE human renal cancer tissue with Fos B antibody. HIER: Boil the paraffin sections in pH8 EDTA for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE human placental tissue with Fos B antibody. HIER: Boil the paraffin sections in pH8 EDTA for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE mouse intestinal tissue with Fos B antibody. HIER: Boil the paraffin sections in pH8 EDTA for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat intestinal tissue with Fos B antibody. HIER: Boil the paraffin sections in pH8 EDTA for 20 minutes and allow to cool prior to staining.
Western blot testing of 1) rat thymus and mouse 2) thymus, 3) ovary, 4) spleen, 5) kidney, 6) RAW264.7 and human 7) HL-60 and 8) ThP-1 lysate with Fos B antibody. Expected molecular weight ~36 kDa.
Flow cytometry testing of human SiHa cells with Fos B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Fos B antibody.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Fos B antibody should be determined by the researcher.
Description:
FOSB, FBJ murine osteosarcoma viral oncogene homolog B, is a protein that, in humans, is encoded by the FOSB gene. FOSB is a member of Fos gene family which consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. The FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. The FOSB gene is mapped to 19q13.32. Delta FOSB is a truncated splice variant of FOSB. Delta FosB has been implicated in the development of drug addiction and control of the reward system in the brain, and is linked to changes in a number of other gene products such as CREB and sirtuins. Delta FosB also regulates the commitment of mesenchymal precursor cells to the adipocyte or osteoblast lineage.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids LPFQTSQDAPPNLTASLFTHSEVQVLGD of human Fos B were used as the immunogen for the Fos B antibody.
Limitation:
This Fos B antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P53539