Galectin 1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R32841
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32841-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Galectin 1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) mouse thymus, 2) mouse kidney, 3) mouse testis, 4) mouse ovary, 5) rat heart, 6) rat skeletal muscle, 7) rat stomach and 8) rat testis tissue lysate with Galectin 1 antibody at 0.5ug/ml. Predicted molecular weight: ~15 kDa.
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IHC testing of FFPE rat kidney tissue with Galectin 1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
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IHC testing of FFPE rat small intestine tissue with Galectin 1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
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IHC testing of FFPE mouse kidney tissue with Galectin 1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
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Western blot testing of 1) mouse thymus, 2) mouse kidney, 3) mouse testis, 4) mouse ovary, 5) rat heart, 6) rat skeletal muscle, 7) rat stomach and 8) rat testis tissue lysate with Galectin 1 antibody at 0.5ug/ml. Predicted molecular weight: ~15 kDa.
IHC testing of FFPE rat kidney tissue with Galectin 1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat small intestine tissue with Galectin 1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE mouse kidney tissue with Galectin 1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,ELISA (Capture; recombinant mouse protein): 0.1-0.5ug/ml (BSA-free format available)
Application Note:
Optimal dilution of the Galectin 1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Description:
Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Galectin-1 expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ T-cells. It is thought to play a role in creating immune tolerance in pregnancy. It has been found that Galectin-1-mediated production of IL6 may assist in augmenting the innate immune response against NiV. Galectin-1 may also be a significant factor that augments the efficiency of the HIV-1 infection process.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant mouse protein corresponding to amino acids A2-E135 was used as the immunogen for the Galectin 1 antibody.
Limitation:
This Galectin 1 antibody is available for research use only.
Localization:
Cytoplasmic, secreted
Purity:
Antigen affinity
Species Reactivity :
Mouse, Rat
Uniprot #:
P16045