Galectin 1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31924
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31924-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Galectin 1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 6
IF/ICC staining of FFPE human U-2 OS cells with Galectin 1 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 6
IHC testing of FFPE human placenta with Galectin 1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
3 / 6
IHC testing of FFPE mouse intestinal cancer with Galectin 1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
4 / 6
IHC testing of FFPE rat kidney with Galectin 1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
5 / 6
Western blot testing of 1) rat testis, 2) mouse spleen, 3) human HeLa and 4) human SMCC lysate with Galectin 1 antibody. Expected molecular weight: ~15 kDa.
6 / 6
Flow cytometry testing of human PC-3 cells with Galectin 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Galectin 1 antibody.
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IF/ICC staining of FFPE human U-2 OS cells with Galectin 1 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human placenta with Galectin 1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE mouse intestinal cancer with Galectin 1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat kidney with Galectin 1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Western blot testing of 1) rat testis, 2) mouse spleen, 3) human HeLa and 4) human SMCC lysate with Galectin 1 antibody. Expected molecular weight: ~15 kDa.
Flow cytometry testing of human PC-3 cells with Galectin 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Galectin 1 antibody.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,IHC (FFPE): 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,ELISA: 0.1-0.5ug/ml (human protein tested); request BSA-free format for coating
Application Note:
Optimal dilution of the Galectin 1 antibody should be determined by the researcher.
Description:
Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Galectin-1 expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ T-cells. It is thought to play a role in creatingimmune tolerance in pregnancy. It has been found that Galectin-1-mediated production of IL6 may assist in augmenting the innate immune response against NiV. Galectin-1 may also be a significant factor that augments the efficiency of the HIV-1 infection process.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 2-135 of human Galectin 1 were used as the immunogen for the Galectin 1 antibody.
Limitation:
This Galectin 1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P09382