Galectin 3 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31507
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31507-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Galectin 3 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) HeLa, 2) SiHa, 3) U-87 MG, 4) RT4, 5) HaCaT, 6) PC-3 and 7) T-47D cell lysate with Galectin 3 antibody. Predicted molecular weight ~26 kDa.
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IHC-P: Galectin 3 antibody testing of human lung cancer tissue. HIER: steam section in pH6 citrate buffer for 20 min.
3 / 6
IHC-P: Galectin 3 antibody testing of human placenta tissue. HIER: steam section in pH6 citrate buffer for 20 min.
4 / 6
Immunofluorescent staining of FFPE human U-2 OS cells with Galectin 3 antibody at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
5 / 6
Immunofluorescent staining of FFPE human SiHa cells with Galectin 3 antibody (green) at and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Flow cytometry testing of human U-2 OS cells with Galectin 3 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Galectin 3 antibody.
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Western blot testing of human 1) HeLa, 2) SiHa, 3) U-87 MG, 4) RT4, 5) HaCaT, 6) PC-3 and 7) T-47D cell lysate with Galectin 3 antibody. Predicted molecular weight ~26 kDa.
IHC-P: Galectin 3 antibody testing of human lung cancer tissue. HIER: steam section in pH6 citrate buffer for 20 min.
IHC-P: Galectin 3 antibody testing of human placenta tissue. HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human U-2 OS cells with Galectin 3 antibody at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human SiHa cells with Galectin 3 antibody (green) at and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human U-2 OS cells with Galectin 3 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Galectin 3 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Galectin 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Galectin 3 (GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, it located on chromosome 14, locus q21?q22. It is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin 3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
3958
Immunogen:
Human partial recombinant protein (AA 139-250) was used as the immunogen for this Galectin 3 antibody.
Limitation:
This Galectin 3 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human