Galectin Antibody

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NSJ Bioreagents
Product Code: NSJ-R30492
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30492-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Galectin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 10
Immunofluorescent staining of FFPE human U-2 OS cells with Galectin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 10
Western blot testing of Galectin antibody and Lane 1: recombinant human protein 10ng; 2: 5ng; 3: 2.5ng.
3 / 10
Western blot testing of human 1) HeLa, 2) MCF7, 3) COLO-320, 4) A549, 5) 22RV1 and 6) A375 cell lysate with Galectin antibody. Expected molecular weight ~14 kDa.
4 / 10
IHC staining of FFPE human tonsil with Galectin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 10
IHC staining of FFPE human glioma with Galectin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 10
IHC staining of FFPE human stomach cancer with Galectin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 10
IHC staining of FFPE human ovarian cancer with Galectin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 10
IHC staining of FFPE human liver cancer with Galectin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 10
IHC staining of frozen human placenta with Galectin antibody.
10 / 10
Flow cytometry testing of human PC-3 cells with Galectin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Galectin antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with Galectin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of Galectin antibody and Lane 1: recombinant human protein 10ng; 2: 5ng; 3: 2.5ng.
Western blot testing of human 1) HeLa, 2) MCF7, 3) COLO-320, 4) A549, 5) 22RV1 and 6) A375 cell lysate with Galectin antibody. Expected molecular weight ~14 kDa.
IHC staining of FFPE human tonsil with Galectin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human glioma with Galectin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human stomach cancer with Galectin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian cancer with Galectin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer with Galectin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of frozen human placenta with Galectin antibody.
Flow cytometry testing of human PC-3 cells with Galectin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Galectin antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunohistochemistry (frozen): 1-2ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Galectin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Galectin 1 is a protein that in humans is encoded by the LGALS1 gene. Galectins belong to a family of related beta-galactoside-binding lectins, also referred to as S-type or S-Lac lectins. Its gene is mapped to 22q12-q13. Galectin 1 was expressed in a subset of slowly dividing subventricular zone astrocytes, which included the neural stem cells. It has been considered as an autocrine negative growth factor that regulates cell proliferation.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human Galectin (NYMAADGDFKIKCVAFD) was used as the immunogen for this Galectin antibody.
Limitation:
This Galectin antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P09382