gamma Catenin Antibody

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NSJ Bioreagents
Product Code: NSJ-R30283
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30283-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the gamma Catenin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human HeLa cells with Gamma Catenin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) 293T, 2) MCF7 and 3) T47D cell lysate with Gamma Catenin antibody. Expected molecular weight: ~86 kDa.
3 / 3
Flow cytometry testing of human A431 cells with Gamma Catenin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Gamma Catenin antibody.
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Immunofluorescent staining of FFPE human HeLa cells with Gamma Catenin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) 293T, 2) MCF7 and 3) T47D cell lysate with Gamma Catenin antibody. Expected molecular weight: ~86 kDa.
Flow cytometry testing of human A431 cells with Gamma Catenin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Gamma Catenin antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the gamma Catenin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Catenin gamma, also known as Junction plakoglobin (JUP) or Plakoglobin (PKGB), is a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. Catenin beta and gamma, vertebrate homologs of Drosophila armadillo, function in cell adhesion and the Wnt signaling pathway. Catenin gamma may have distinct roles in Wnt signaling and cancer via differential effects on downstream target genes.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of human gamma Catenin (LLATQVEGQATNLQRLAE) was used as the immunogen for this gamma Catenin antibody.
Limitation:
This gamma Catenin antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P14923