GAPDH Loading Control Antibody [Biotin Conjugate]

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NSJ Bioreagents
Product Code: NSJ-R34262BTN
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R34262BTN-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Isotype: Goat Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Porcine (pig)
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store the GAPDH loading control antibody at -200.

Images

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Western blot testing of HEK293 lysate with GAPDH loading control antibody at 0.5ug/ml. Predicted molecular weight ~36kDa. An NAP blocker was used in place of non-fat milk as blocking solution and diluent. (1)
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Western blot testing of HEK293 lysate with GAPDH loading control antibody at 0.5ug/ml. Predicted molecular weight ~36kDa. An NAP blocker was used in place of non-fat milk as blocking solution and diluent. (1)

Documents

Further Information

Application Details :
Western blot: 0.3-1ug/ml,ELISA (peptide) LOD: 1:64000
Application Note:
Optimal dilution of the GAPDH loading control antibody should be determined by the researcher.

1. Do not use a blocking agent or diluent that contains biotin (including milk and some BSA preparations) when testing with biotinylated antibodies. A non-animal protein (NAP) blocker is recommended.

Description:
Additional name(s) for this target protein: Glyceraldehyde-3-phosphate dehydrogenase; GAPDH
Format :
Biotin Conjugate
Formulation :
0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Gene ID #:
2597
Immunogen:
Amino acids HQVVSSDFNSDT were used as the immunogen for this GAPDH loading control antibody.
Limitation:
This GAPDH loading control antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat, Pig
Species Reactivity (Predicted):
Dog