GAT-1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30811
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30811-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the GAT-1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of GAT-1 antibody and Lane 1: rat brain; 2: mouse brain tissue lysate. Predicted size: 67~80 depending on level of glycosylation. The ~35KD band in uncharacterized.~
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IHC-P: GAT-1 antibody testing of rat brain tissue
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Western blot testing of GAT-1 antibody and Lane 1: rat brain; 2: mouse brain tissue lysate. Predicted size: 67~80 depending on level of glycosylation. The ~35KD band in uncharacterized.~
IHC-P: GAT-1 antibody testing of rat brain tissue

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the GAT-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
SLC6A1 (Solute Carrier family 6(Neurotransmitter transporter, Gaba), Member 1), also called GABATR, GABATHG and GAT-1, encodes a gamma-aminobutyric acid (GABA) transporter, which removes GABA from the synaptic cleft. Zomot et al.(2007) showed that introduction of a negatively charged amino acid at or near one of the two putative sodium binding sites of GAT-1 from rat brain renders both net flux and exchange of GABA largely chloride independent. By in vitro functional expression studies, the GAT-1 insertion promoter (two copies of the sequence) had significantly greater promoter activity than the noninsertion (one copy of the sequence) promoter, which in turn had greater activity than a promoterless control. Studies of postmortem hippocampus from 69 African American individuals showed that overall expression decreased with age.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human GABA Transporter 1 (WFYGVNRFYDNIQEM) was used as the immunogen for this GAT-1 antibody (100% homologous in human, mouse and rat).
Limitation:
This GAT-1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P30531