GATA2 Antibody

Western blot: 0.5-1ug/ml

The stated application concentrations are suggested starting amounts. Titration of the GATA2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

The GATA family of transcription factors, which contain zinc fingers in their DNA binding domain, have emerged as candidate regulators of gene expression in hematopoietic cells. Ciciotte et al.(1997) mapped the mouse GATA2 gene to chromosome 6 by study of DNA from an interspecific backcross panel. They pointed out that the human gene had been mapped to chromosome 3 by Dorfman et al.(1992) using DNA from a panel of 12 rodent/human hybrids containing various human chromosomes and applying Southern blot analysis. By exon trapping using a PAC contig spanning a breakpoint region associated with myeloid leukemia, Wieser et al.(2000) mapped the GATA2 gene to chromosome 3q21. GATA2 is transcribed from telomere to centromere. GATA2 is expressed in hematopoietic progenitors, including early erythroid cells, mast cells, and megakaryocytes, and also in nonhematopoietic embryonic stem cells.

Antigen affinity purified

0.5mg/ml if reconstituted with 0.2ml sterile DI water

An amino acid sequence from the N-terminus of human GATA2 (DVFFNHLDSQGNPYY) was used as the immunogen for this GATA2 antibody (100% homologous in human, mouse and rat).

This GATA2 antibody is available for research use only.

Antigen affinity

Human, Mouse, Rat

P23769