GDNF Antibody

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NSJ Bioreagents
Product Code: NSJ-R30537
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30537-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the GDNF antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human lung cancer with GDNF antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE rat brain with GDNF antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
3 / 3
Western blot testing of GDNF antibody and Lane 1: recombinant human protein 10ng; 2: 5ng; 3: 2.5ng; 4: 1.25ng
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IHC staining of FFPE human lung cancer with GDNF antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain with GDNF antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Western blot testing of GDNF antibody and Lane 1: recombinant human protein 10ng; 2: 5ng; 3: 2.5ng; 4: 1.25ng

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the GDNF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Glial cell-derived neurotrophic factor, is a protein that, in humans, is encoded by the GDNF gene. It is a small protein that potently promotes the survival of many types of neurons and a founding member of the GDNF family of ligands(GFL). The gene is mapped to human chromosome 5p13.1-p12 by fluorescence in situ hybridization(FISH). This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosisof motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET(rearranged during transfection) protooncogene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the middle region of human GDNF (FRYCSGSCDAAETTYDK) was used as the immunogen for this GDNF antibody.
Limitation:
This GDNF antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P39905