Gelsolin Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-R31221
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31221-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Gelsolin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
Western blot testing of 1) human HeLa, 2) (h) THP1, 3) (h) A549, 4) monkey COS-7, 5) (h) Caco-2, 6) (h) K562 and 7) (h) HL-60 lysate with Gelsolin antibody at 0.5ug/ml. Predicted molecular weight ~86 kDa.
2 / 5
IHC-P: Gelsolin antibody testing of human intestine cancer tissue. HIER: steamed with pH6 citrate buffer.
3 / 5
IHC-P: Gelsolin antibody testing of human lung cancer tissue. HIER: steamed with pH6 citrate buffer.
4 / 5
ICC testing of Gelsolin antibody and NIH3T3 cells
5 / 5
IHC-P: Gelsolin antibody testing of rat heart tissue. HIER: steamed with pH6 citrate buffer.
❮❯

Western blot testing of 1) human HeLa, 2) (h) THP1, 3) (h) A549, 4) monkey COS-7, 5) (h) Caco-2, 6) (h) K562 and 7) (h) HL-60 lysate with Gelsolin antibody at 0.5ug/ml. Predicted molecular weight ~86 kDa.
IHC-P: Gelsolin antibody testing of human intestine cancer tissue. HIER: steamed with pH6 citrate buffer.
IHC-P: Gelsolin antibody testing of human lung cancer tissue. HIER: steamed with pH6 citrate buffer.
ICC testing of Gelsolin antibody and NIH3T3 cells
IHC-P: Gelsolin antibody testing of rat heart tissue. HIER: steamed with pH6 citrate buffer.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Gelsolin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Gelsolin also known as GNS is an actin-binding protein that is a key regulator of actin filament assembly and disassembly. It is one of the most potent members of the actin-severing gelsolin/villin superfamily. The gene was assigned to human chromosome 9q33.2. Gelsolin is also known as brevin, or actin-depolymerizing factor; it is the principal intracellular and extracellular actin-severing protein. Gelsolin and Gc protein together constitute the extracellular actin-scavenger system which prevents the toxic effects of actin release into the extracellular space under circumstances of cell necrosis. It may have therapeutic potential as a mucolytic agent in CF patients. The antiapoptotic activity of Gelsolin seems to prevent a step leading to cytochrome c release from the mitochondria into the cytosol.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 763-775 (WDDDYWSVDPLDR-human) were used as the immunogen for this Gelsolin antibody (100% rat homology).
Limitation:
This Gelsolin antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P06396