GFAP Antibody

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NSJ Bioreagents
Product Code: NSJ-R31508
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31508-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the GFAP antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of GFAP antibody and Lane 1: rat brain; 2: mouse brain; 3: U87; 4: SHG-44 (human glioma line); 5: NEURO; 6: HeLa lysate
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IHC-P: GFAP antibody testing of human brain cancer
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IHC-P: GFAP antibody testing of mouse brain tissue
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IHC-P: GFAP antibody testing of rat brain tissue
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Immunofluorescent staining of FFPE mouse brain with GFAP antibody (red) and DAPI nuclear counterstain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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Western blot testing of GFAP antibody and Lane 1: rat brain; 2: mouse brain; 3: U87; 4: SHG-44 (human glioma line); 5: NEURO; 6: HeLa lysate
IHC-P: GFAP antibody testing of human brain cancer
IHC-P: GFAP antibody testing of mouse brain tissue
IHC-P: GFAP antibody testing of rat brain tissue
Immunofluorescent staining of FFPE mouse brain with GFAP antibody (red) and DAPI nuclear counterstain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence (FFPE): 1-2ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the GFAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Glial fibrillary acidic protein is a protein that is encoded by the GFAP gene in humans. It is an intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS) including astrocytes, and ependymal cells. It is mapped to 17q21.31. It is closely related to its non-epithelial family members, vimentin, desmin, and peripherin, which are all involved in the structure and function of the cells cytoskeleton. GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells. This gene has been shown to play a role in mitosis by adjusting the filament network present in the cell. It is necessary for many critical roles in the CNS. It also plays a role in astrocyte-neuron interactions as well as cell-cell communication.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
2670
Immunogen:
Human partial recombinant protein (AA 93-432) was used as the immunogen for this GFAP antibody.
Limitation:
This GFAP antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat