Gli3 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-R30859
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30859-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the Gli3 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 1
Western blot testing of Gli3 antibody and Lane 1: rat testis; 2: human A549; 3: (h) SW620 cell lysate
❮❯

Western blot testing of Gli3 antibody and Lane 1: rat testis; 2: human A549; 3: (h) SW620 cell lysate

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml (1)
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Gli3 antibody may be required due to differences in protocols and secondary/substrate sensitivity. 1. There are two isoforms of Gli-kruppel family member 3: the 170-190KD full length form and a truncated ~80KD form. This antibody will detect both, depending on the testing material.
Description:
Gli-kruppel family member 3 is a member of the zinc finger family related to Kruppel, a protein family that is known to regulate development in Drosophila. The gene is expressed as an 8.5-kb mRNA in tissues such as testis, myometrium, placenta, and lung, and the protein product (relative molecular mass, 190,000D) shows sequence-specific DNA binding. Gli3 is homologous to the Drosophila cubitus interruptus (ci) gene product(Ci), which regulates the Patched (pct), Gooseberry (gsb), and Decapentaplegic (dpp) genes. PKA-dependent processing of vertebrate Gli3 in developing limb generates a potent repressor in a manner antagonized by apparent long-range signaling from posteriorly localized Sonic hedgehog protein. The high relative abundance and potency of Gli3 repressor suggested specialization of the protein and its products for negative hedgehog pathway regulation.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of human Gli-kruppel family member 3 (SNEDESPGQTYHRERRNA) was used as the immunogen for this Gli3 antibody.
Limitation:
This Gli3 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P10071