GLO1 Antibody / Glyoxalase I

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NSJ Bioreagents
Product Code: NSJ-RQ4238
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4238-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the GLO1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 6
Western blot testing of human 1) HeLa, 2) placenta, 3) MCF7, 4) COLO320, 5) 22RV1, 6) HepG2, 7) A431 and 8) U937 lysate with GLO1 antibody at 0.5ug/ml. Predicted molecular weight ~21 kDa.
2 / 6
Western blot testing of rat 1) brain, 2) testis, 3) spleen, 4) thymus and mouse 5) brain, 6) testis, 7) thymus and 8) liver lysate with GLO1 antibody at 0.5ug/ml. Predicted molecular weight ~21 kDa.
3 / 6
IHC testing of FFPE human lung cancer tissue with GLO1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 6
IHC testing of FFPE human breast cancer tissue with GLO1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 6
IHC testing of FFPE rat small intestine tissue with GLO1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
6 / 6
IHC testing of FFPE rat spleen tissue with GLO1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human 1) HeLa, 2) placenta, 3) MCF7, 4) COLO320, 5) 22RV1, 6) HepG2, 7) A431 and 8) U937 lysate with GLO1 antibody at 0.5ug/ml. Predicted molecular weight ~21 kDa.
Western blot testing of rat 1) brain, 2) testis, 3) spleen, 4) thymus and mouse 5) brain, 6) testis, 7) thymus and 8) liver lysate with GLO1 antibody at 0.5ug/ml. Predicted molecular weight ~21 kDa.
IHC testing of FFPE human lung cancer tissue with GLO1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human breast cancer tissue with GLO1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE rat small intestine tissue with GLO1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE rat spleen tissue with GLO1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the GLO1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Lactoylglutathione lyase in humans is encoded by the GLO1 gene. The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids A2-M184 was used as the immunogen for the GLO1 antibody.
Limitation:
This GLO1 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear, membrane
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q04760