GLUR2 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31618
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31618-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the GLUR2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human T-47D cells with GLUR2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE mouse brain tissue with GLUR2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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IHC staining of FFPE mouse brain with GLUR2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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IHC staining of FFPE rat brain with GLUR2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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IHC staining of frozen rat brain tissue with GLUR2 antibody.
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Western blot testing of 1) rat brain, 2) rat C6 and 3) mouse brain lysate with GLUR2 antibody. Predicted molecular weight ~99 kDa.
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Flow cytometry testing of human U-87 MG cells with GLUR2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GLUR2 antibody.
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Immunofluorescent staining of FFPE human T-47D cells with GLUR2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE mouse brain tissue with GLUR2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE mouse brain with GLUR2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE rat brain with GLUR2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of frozen rat brain tissue with GLUR2 antibody.
Western blot testing of 1) rat brain, 2) rat C6 and 3) mouse brain lysate with GLUR2 antibody. Predicted molecular weight ~99 kDa.
Flow cytometry testing of human U-87 MG cells with GLUR2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GLUR2 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence: 2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the GLUR2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Glutamate receptor 2, also known as GLUR2, is a protein that in humans is encoded by the GRIA2 gene. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. The crystal structures of the GLUR2 ligand-binding core in the apo state and in the presence of the antagonist DNQX, the partial agonist kainate, and the full agonists AMPA and glutamate. GLUR2 plays a major role in depression at synapses in which glutamate remains in the synaptic cleft for prolonged periods of time during normal operation of the synapse. Overexpression increases dendritic spine size and density in hippocampal neurons, and more remarkably, induces spine formation in GABA-releasing interneurons that normally lack spines.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
14800
Immunogen:
Human partial recombinant protein (AA 25-360) was used as the immunogen for this GLUR2 antibody.
Limitation:
This GLUR2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat