GLUT1 Antibody / SLC2A1

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NSJ Bioreagents
Product Code: NSJ-R31941
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31941-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the SLC2A1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human HepG2 cells with SLC2A1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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ICC testing of human A549 cells with SLC2A1 antibody.
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IHC testing of FFPE human placenta with SLC2A1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
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IHC testing of frozen human placenta with SLC2A1 antibody.
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IHC testing of FFPE mouse brain with SLC2A1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
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IHC testing of FFPE rat brain with SLC2A1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
7 / 15
IHC testing of FFPE human placenta with SLC2A1 antibody. HIER: Boil the paraffin sections in pH 8 EDTA for 20 minutes and allow to cool prior to staining.
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IHC testing of FFPE human placenta with SLC2A1 antibody. HIER: Boil the paraffin sections in pH 8 EDTA for 20 minutes and allow to cool prior to staining.
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IHC testing of FFPE human breast cancer with SLC2A1 antibody. HIER: Boil the paraffin sections in pH 8 EDTA for 20 minutes and allow to cool prior to staining.
10 / 15
IHC testing of FFPE mouse brain with SLC2A1 antibody. HIER: Boil the paraffin sections in pH 8 EDTA for 20 minutes and allow to cool prior to staining.
11 / 15
IHC testing of FFPE rat brain with SLC2A1 antibody. HIER: Boil the paraffin sections in pH 8 EDTA for 20 minutes and allow to cool prior to staining.
12 / 15
IHC testing of FFPE rat brain with SLC2A1 antibody. HIER: Boil the paraffin sections in pH 8 EDTA for 20 minutes and allow to cool prior to staining.
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Western blot testing of 1) human placenta, 2) human A549, 3) mouse brain, 4) rat PC-12 and 5) mouse NIH 3T3 lysate wtih SLC2A1 antibody. Predicted molecular weight ~55 kDa.
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Western blot testing of 1) rat brain, 2) mouse brain and 3) mouse NIH 3T3 lysate wtih SLC2A1 antibody. Predicted molecular weight ~55 kDa.
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Flow cytometry testing of human A431 cells with SLC2A1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SLC2A1 antibody.
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Immunofluorescent staining of FFPE human HepG2 cells with SLC2A1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
ICC testing of human A549 cells with SLC2A1 antibody.
IHC testing of FFPE human placenta with SLC2A1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of frozen human placenta with SLC2A1 antibody.
IHC testing of FFPE mouse brain with SLC2A1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat brain with SLC2A1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE human placenta with SLC2A1 antibody. HIER: Boil the paraffin sections in pH 8 EDTA for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE human placenta with SLC2A1 antibody. HIER: Boil the paraffin sections in pH 8 EDTA for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE human breast cancer with SLC2A1 antibody. HIER: Boil the paraffin sections in pH 8 EDTA for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE mouse brain with SLC2A1 antibody. HIER: Boil the paraffin sections in pH 8 EDTA for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat brain with SLC2A1 antibody. HIER: Boil the paraffin sections in pH 8 EDTA for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat brain with SLC2A1 antibody. HIER: Boil the paraffin sections in pH 8 EDTA for 20 minutes and allow to cool prior to staining.
Western blot testing of 1) human placenta, 2) human A549, 3) mouse brain, 4) rat PC-12 and 5) mouse NIH 3T3 lysate wtih SLC2A1 antibody. Predicted molecular weight ~55 kDa.
Western blot testing of 1) rat brain, 2) mouse brain and 3) mouse NIH 3T3 lysate wtih SLC2A1 antibody. Predicted molecular weight ~55 kDa.
Flow cytometry testing of human A431 cells with SLC2A1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SLC2A1 antibody.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunocytochemistry: 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the SLC2A1 antibody should be determined by the researcher.
Description:
GLUT1, also known as SLC2A1, is a major glucose transporter in the mammalian blood-brain barrier whose gene is mapped to 1p35-p31.3 and contains 10 exons. It is present at high levels in primate erythrocytes and brain endothelial cells. Not only can transport dehydroascorbic acid (the oxidized form of vitamin C) into the brain, GLUT1 is also likely to contribute to HTLV-associated disorders through interacting with HTLV envelope glycoproteins. Functionally, GLUT1 deficiency causes a decrease in embryonic glucose uptake and apoptosis, which may be involved in diabetic embryopathy, by contrast, an increased expression of GLUT1 in some malignant tumors may suggest a role for glucose-derivative tracers to detect in vivo thyroid cancer metastases by positron-emission tomography scanning.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 92-492 of human SLC2A1 were used as the immunogen for the SLC2A1 antibody.
Limitation:
This SLC2A1 antibody is available for research use only.
Localization:
Cytoplasmic, membrane
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P11166