GNB1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R32535
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32535-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the GNB1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 7
Western blot testing of 1) rat liver, 2) mouse heart and 3) human HeLa lysate with GNB1 antibody at 0.5ug/ml. Predicted/observed molecular weight: ~37 kDa.
2 / 7
IHC testing of FFPE human liver cancer tissue with GNB1 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
3 / 7
IHC testing of FFPE mouse kidney with GNB1 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
4 / 7
IHC testing of FFPE rat kidney with GNB1 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
5 / 7
IHC testing of FFPE human placental tissue with GNB1 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
6 / 7
IF/ICC staining of FFPE human A549 cells with GNB1 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
7 / 7
Flow cytometry testing of human U937 cells with GNB1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GNB1 antibody.
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Western blot testing of 1) rat liver, 2) mouse heart and 3) human HeLa lysate with GNB1 antibody at 0.5ug/ml. Predicted/observed molecular weight: ~37 kDa.
IHC testing of FFPE human liver cancer tissue with GNB1 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE mouse kidney with GNB1 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE rat kidney with GNB1 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human placental tissue with GNB1 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IF/ICC staining of FFPE human A549 cells with GNB1 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human U937 cells with GNB1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GNB1 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence/Immunocytochemistry (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 is a protein that in humans is encoded by the GNB1 gene. Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. This gene uses alternative polyadenylation signals.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 2-42 (SELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGR) from the human protein were used as the immunogen for the GNB1 antibody.
Limitation:
This GNB1 antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P62873