GRIK1 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-R31621
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31621-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the GRIK1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 7
Western blot testing of GRIK1 antibody and Lane 1: mouse brain; 2: mouse brain; 3: human SHG-44 (glioma) lysate. Predicted/observed size ~104KD
2 / 7
Western blot testing of GRIK1 antibody and recombinant human protein (0.5ng)
3 / 7
Western blot testing of 1) rat brain and 2) mouse brain lysate with GRIK1 antibody. Predicted molecular weight: ~104/150+ kDa (unmodified/glycosylated).
4 / 7
IHC staining of FFPE human glioma with GRIK1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
5 / 7
IHC staining of FFPE mouse brain with GRIK1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
6 / 7
IHC staining of FFPE human lung cancer with GRIK1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
7 / 7
IHC staining of FFPE human thyroid cancer with GRIK1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
❮❯

Western blot testing of GRIK1 antibody and Lane 1: mouse brain; 2: mouse brain; 3: human SHG-44 (glioma) lysate. Predicted/observed size ~104KD
Western blot testing of GRIK1 antibody and recombinant human protein (0.5ng)
Western blot testing of 1) rat brain and 2) mouse brain lysate with GRIK1 antibody. Predicted molecular weight: ~104/150+ kDa (unmodified/glycosylated).
IHC staining of FFPE human glioma with GRIK1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE mouse brain with GRIK1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with GRIK1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human thyroid cancer with GRIK1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the GRIK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Glutamate receptor, ionotropic, kainate 1, also known as GLUR5, is a protein that in humans is encoded by the GRIK1 gene. It is mapped to 21q21.3. This gene encodes one of the many ionotropic glutamate receptor (GluR) subunits that function as a ligand-gated ion channel. The specific GluR subunit encoded by this gene is of the kainate receptor subtype. Receptor assembly and intracellular trafficking of ionotropic glutamate receptors are regulated by RNA editing and alternative splicing. These receptors mediate excitatory neurotransmission and are critical for normal synaptic function. Exons of this gene are interspersed with exons from the C21or f41 gene, which is transcribed in the same orientation as this gene but does not seem to encode a protein.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
2897
Immunogen:
Human partial recombinant protein (AA 271-450) was used as the immunogen for this GRIK1 antibody.
Limitation:
This GRIK1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat