GRP75 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30886
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30886-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Grp75 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
Western blot testing of 1) human HeLa, 2) human A549, 3) human Jurkat, 4) human HepG2, 5) rat testis, 6) mouse testis and 7) mouse NIH 3T3 cell lysate with GRP75 antibody. Expected molecular weight: 70~75 kDa.
2 / 5
IHC-P: GRP75 antibody testing of human lung cancer tissue. HIER: steamed with pH6 citrate buffer.
3 / 5
IHC-P: GRP75 antibody testing of rat liver tissue. HIER: steamed with pH6 citrate buffer.
4 / 5
ICC testing of A549 cells
5 / 5
IHC staining of frozen rat liver tissue with GRP75 antibody.
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Western blot testing of 1) human HeLa, 2) human A549, 3) human Jurkat, 4) human HepG2, 5) rat testis, 6) mouse testis and 7) mouse NIH 3T3 cell lysate with GRP75 antibody. Expected molecular weight: 70~75 kDa.
IHC-P: GRP75 antibody testing of human lung cancer tissue. HIER: steamed with pH6 citrate buffer.
IHC-P: GRP75 antibody testing of rat liver tissue. HIER: steamed with pH6 citrate buffer.
ICC testing of A549 cells
IHC staining of frozen rat liver tissue with GRP75 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Grp75 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Heat shock 70kDa protein 9, also known as GRP75, mot-2, mthsp75, PBP74, HSPA9B, and Mortalin, is a highly conserved member of the HSP70 family of proteins. It functions as a chaperone in the mitochondria, cytoplasm, and centrosome. The HSPA9 gene is mapped to chromosome 5q31.2 based on an alignment of the HSPA9 sequence with the genomic sequence. Knockdown of the protein in erythroid cultures was associated with an increased number of cells in the G0/G1 phase of the cell cycle and accelerated apoptosis. Knockdown of the protein in mouse bone marrow cells followed by transplantation into wildtype recipients also resulted in loss of erythroid cell number. Haploinsufficiency for GRP75 may contribute to abnormal hematopoiesis in myelodysplastic syndromes with deletions spanning chromosome 5q31.2. This protein plays a role in the control of cell proliferation. It may also act as a chaperone.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human GRP75 (DTETKMEEFKDQLPADECN) was used as the immunogen for this GRP75 antibody (100% homologous in human, mouse and rat).
Limitation:
This GRP75 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P38646