GST pi Antibody (GST3)

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NSJ Bioreagents
Product Code: NSJ-R30217
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30217-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Application: Western Blot (WB)
Storage:
After reconstitution, the GST pi antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human ovarian cancer tissue with GST pi antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE moderately differentiated human adenocarcinoma of the cervical canal tissue with GST pi antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
IHC staining of FFPE moderately differentiated highly differentiated human adenocarcinoma of the colon tissue with GST pi antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 4
Western blot testing of 1) human HeLa, 2) human 293T, 3) human Jurkat, 4) rat lung 5) mouse lung and 6) mouse liver tissue lysate with GST pi antibody. Predicted molecular weight ~23 kDa.
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IHC staining of FFPE human ovarian cancer tissue with GST pi antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE moderately differentiated human adenocarcinoma of the cervical canal tissue with GST pi antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE moderately differentiated highly differentiated human adenocarcinoma of the colon tissue with GST pi antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human 293T, 3) human Jurkat, 4) rat lung 5) mouse lung and 6) mouse liver tissue lysate with GST pi antibody. Predicted molecular weight ~23 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the GST pi antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Glutathione S-transferases pi, also known as GST pi and GST3, is present in all tissues and cells, with the exception of red cells, in which only erythrocyte GST(GSTe) is observed. The gene has 7 exons and 6 introns contained within approximately 2.8 kilobases. It is mapped to chromosome 11 . Placental glutathione-S-transferase-pi mRNA is abundantly expressed in human skin. GST pi does not contribute in vivo to the formation of glutathione conjugates of acetaminophen but plays a novel and unexpected role in the toxicity of this compound.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids PEYVNLPINGNGKQ were used as the immunogen for this GST pi antibody.
Limitation:
This GST pi antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Uniprot #:
P09211