HDAC4 Antibody

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NSJ Bioreagents
Product Code: NSJ-R32738
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32738-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the HDAC4 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) rat brain, 2) rat lung, 3) rat testis, 4) mouse lung, 5) mouse brain, 6) mouse testis and 7) human HepG2 lysate with HDAC4 antibody at 0.5ug/ml. Expected molecular weight: ~140 kDa (full length), ~95 kDa (truncated).
2 / 3
Flow cytometry testing of human ThP-1 cells with HDAC4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HDAC4 antibody.
3 / 3
Flow cytometry testing of human SiHa cells with HDAC4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HDAC4 antibody.
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Western blot testing of 1) rat brain, 2) rat lung, 3) rat testis, 4) mouse lung, 5) mouse brain, 6) mouse testis and 7) human HepG2 lysate with HDAC4 antibody at 0.5ug/ml. Expected molecular weight: ~140 kDa (full length), ~95 kDa (truncated).
Flow cytometry testing of human ThP-1 cells with HDAC4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HDAC4 antibody.
Flow cytometry testing of human SiHa cells with HDAC4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HDAC4 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the HDAC4 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Description:
Histone deacetylase 4, also known as HDAC4, is a protein that in humans is encoded by the HDAC4 gene. It is mapped to 2q37.3. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids Q4-Q213 from the human protein were used as the immunogen for the HDAC4 antibody.
Limitation:
This HDAC4 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P56524