Hemoglobin Antibody (subunit alpha)

NSJ Bioreagents
Product Code: NSJ-R32748
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32748-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Hemoglobin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
IHC testing of FFPE human placental tissue with Hemoglobin antibody. Required HIER: steam section in pH8 EDTA buffer for 20 min and allow to cool prior to testing.
2 / 4
IHC testing of FFPE human tonsil tissue with Hemoglobin antibody. Required HIER: steam section in pH8 EDTA buffer for 20 min and allow to cool prior to testing.
3 / 4
Western blot testing of human placental tissue lysate with Hemoglobin antibody. Predicted molecular weight ~15 kDa.
4 / 4
Flow cytometry testing of fixed and permeabilized human K562 cells with Hemoglobin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Hemoglobin antibody.

IHC testing of FFPE human placental tissue with Hemoglobin antibody. Required HIER: steam section in pH8 EDTA buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE human tonsil tissue with Hemoglobin antibody. Required HIER: steam section in pH8 EDTA buffer for 20 min and allow to cool prior to testing.
Western blot testing of human placental tissue lysate with Hemoglobin antibody. Predicted molecular weight ~15 kDa.
Flow cytometry testing of fixed and permeabilized human K562 cells with Hemoglobin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Hemoglobin antibody.

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the Hemoglobin antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Description:
The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some non-deletion alpha thalassemias have also been reported.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids V2-R142 from the human alpha subunit were used as the immunogen for the Hemoglobin antibody.
Limitation:
This Hemoglobin antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Rat
Uniprot #:
P69905