HGD Antibody / Homogentisate 1,2-dioxygenase

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ4413
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4413-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the HGD antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 3
Western blot testing of 1) human HepG2, 2) human A549, 3) rat liver, 4) rat kidney, 5) mouse liver and 6) mouse kidney tissue with HGD antibody at 0.5ug/ml. Predicted molecular weight ~50 kDa.
2 / 3
IHC testing of FFPE human liver cancer tissue with HGD antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 3
IHC testing of FFPE human prostate cancer tissue with HGD antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
❮❯

Western blot testing of 1) human HepG2, 2) human A549, 3) rat liver, 4) rat kidney, 5) mouse liver and 6) mouse kidney tissue with HGD antibody at 0.5ug/ml. Predicted molecular weight ~50 kDa.
IHC testing of FFPE human liver cancer tissue with HGD antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human prostate cancer tissue with HGD antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Direct ELISA: 0.1-0.5ug/ml (recombinant human protein) (BSA-free format available)
Application Note:
Optimal dilution of the HGD antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
The HGD gene encodes homogentisate 1,2-dioxygenase, an enzyme involved in the catabolism of phenylalanine and tyrosine. This enzyme is involved in the catabolism of the amino acids tyrosine and phenylalanine. Mutations in this gene are the cause of the autosomal recessive metabolism disorder alkaptonuria. This gene is mapped to chromosome 3q21-q23 by a preliminary PCR screen of hamster/human somatic cell hybrid genomic DNA samples and by fluorescence in situ hybridization.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human partial recombinant protein corresponding to amino acids D374-N445 was used as the immunogen for the HGD antibody.
Limitation:
This HGD antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q93099