HLTF Antibody / SMARCA3

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NSJ Bioreagents
Product Code: NSJ-R32538
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32538-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the HLTF antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human A549 cells with HLTF antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human HeLa, 2) human PANC1, 3) human A549, 4) rat heart, 5) rat kidney, 6) mouse heart and 7) mouse kidney tissue lysate with HLTF antibody at 0.5ug/ml. Predicted molecular weight: ~114 kDa.
3 / 3
Flow cytometry testing of human Caco-2 cells with HLTF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HLTF antibody.
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Immunofluorescent staining of FFPE human A549 cells with HLTF antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human PANC1, 3) human A549, 4) rat heart, 5) rat kidney, 6) mouse heart and 7) mouse kidney tissue lysate with HLTF antibody at 0.5ug/ml. Predicted molecular weight: ~114 kDa.
Flow cytometry testing of human Caco-2 cells with HLTF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HLTF antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
Helicase-like transcription factor is an enzyme that in humans is encoded by the HLTF gene. This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids S911-L1009 from the human protein were used as the immunogen for the HLTF antibody.
Limitation:
This HLTF antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q14527