hnRNP F Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-R32625
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32625-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the hnRNP F antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 3
Western blot testing of 1) rat liver, 2) mouse spleen and 3) human HeLa cell lysate with hnRNP F antibody at 0.5ug/ml. Predicted molecular weight ~46 kDa.
2 / 3
IHC testing of FFPE human breast cancer with hnRNP F antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
3 / 3
IHC testing of FFPE human intestinal cancer with hnRNP F antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
❮❯

Western blot testing of 1) rat liver, 2) mouse spleen and 3) human HeLa cell lysate with hnRNP F antibody at 0.5ug/ml. Predicted molecular weight ~46 kDa.
IHC testing of FFPE human breast cancer with hnRNP F antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human intestinal cancer with hnRNP F antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the hnRNP F antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
Heterogeneous nuclear ribonucleoprotein F is a protein that in humans is encoded by the HNRNPF gene. This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 23-53 (SVEDVQNFLSDCTIHDGAAGVHFIYTREGRQ-human) were used as the immunogen for the hnRNP F antibody.
Limitation:
This antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P52597