hnRNP L Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-R32619
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32619-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
After reconstitution, the hnRNP L antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Western blot testing of human 1) PANC and 2) Jurkat cell lysate with hnRNP L antibody at 0.5ug/ml. Predicted molecular weight ~64 kDa.
2 / 4
IF/ICC staining of FFPE human U-2 OS cells with hnRNP L antibody (green) at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
3 / 4
IHC staining of FFPE mouse brain with hnRNP L antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
4 / 4
IHC staining of FFPE rat brain with hnRNP L antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
❮❯

Western blot testing of human 1) PANC and 2) Jurkat cell lysate with hnRNP L antibody at 0.5ug/ml. Predicted molecular weight ~64 kDa.
IF/ICC staining of FFPE human U-2 OS cells with hnRNP L antibody (green) at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE mouse brain with hnRNP L antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE rat brain with hnRNP L antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Immunofluorescence/Immunocytochemistry: 2-4ug/ml
Application Note:
Optimal dilution of the hnRNP L antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
Heterogeneous nuclear ribonucleoprotein L is a protein that in humans is encoded by the HNRNPL gene. Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. HNRNPL is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. It is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions. Two transcript variants encoding different isoforms have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 546-571 (LLEWESKSDALETLGFLNHYQMKNPN-human) were used as the immunogen for the hnRNP L antibody.
Limitation:
This hnRNP L antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P14866