HSD17B1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30694
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30694-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the HSD17B1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) human T-47D, 2) human 293T and 3) rat C6 cell lysate with HSD17B1 antibody. Predicted molecular weight ~35 kDa.
2 / 5
IHC-P: HSD17B1 antibody testing of human placenta tissue
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ICC testing of HeLa cells
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IHC-P: HSD17B1 antibody testing of rat ovary tissue
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IHC-P testing of rat liver tissue
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Western blot testing of 1) human T-47D, 2) human 293T and 3) rat C6 cell lysate with HSD17B1 antibody. Predicted molecular weight ~35 kDa.
IHC-P: HSD17B1 antibody testing of human placenta tissue
ICC testing of HeLa cells
IHC-P: HSD17B1 antibody testing of rat ovary tissue
IHC-P testing of rat liver tissue

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the HSD17B1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
17-beta-Hydroxysteroid dehydrogenas I, also known as 17-beta-hydroxysteroid dehydrogenase or 17-ketosteroid reductase, is an enzyme that is responsible for the interconversion of estrone(E1) and estradiol(E2) as well as the interconversion of androstenedione and testosterone. HSD17B1 gene expression is detected in 34 of 42 (81%) adenomas in all tumor subtypes; HSD17B2 mRNA is detected in 18 of 42(43%) adenomas but not in prolactinomas. Human estrogenic HSD17B1 is an NADP(H)-preferring enzyme. It possesses 11- and 4-fold higher specificity toward NADP(H) over NAD(H) for oxidation and reduction, respectively, as demonstrated by kinetic studies. All four isoforms are variably expressed in human anterior pituitary adenomas, which also show HSD17B enzyme activity, suggesting that it may play an important role in regulating the local cellular levels of estradiol.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 29-43 (QSFKVYATLRDLKTQ-human) were used as the immunogen for this HSD17B1 antibody.
Limitation:
This HSD17B1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P14061