HSF2 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-R30687
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30687-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the HSF2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
Immunofluorescent staining of FFPE human U-2 OS cells with HSF2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 5
IHC testing of FFPE rat brain tissue with HSF2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
3 / 5
IHC testing of FFPE rat brain tissue with HSF2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
4 / 5
Western blot testing of HSF2 antibody and Lane 1: rat kidney; 2: rat spleen; 3: human 293T; 4: (h) MCF-7; 5: (h) Jurkat; 6: (h) A549; 7: (h) CEM lysate; Predicted molecular weight ~60 kDa.
5 / 5
Flow cytometry testing of human 293T cells with HSF2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HSF2 antibody.
❮❯

Immunofluorescent staining of FFPE human U-2 OS cells with HSF2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE rat brain tissue with HSF2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat brain tissue with HSF2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Western blot testing of HSF2 antibody and Lane 1: rat kidney; 2: rat spleen; 3: human 293T; 4: (h) MCF-7; 5: (h) Jurkat; 6: (h) A549; 7: (h) CEM lysate; Predicted molecular weight ~60 kDa.
Flow cytometry testing of human 293T cells with HSF2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HSF2 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the HSF2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Heat shock transcription factor 2 is a protein that binds specifically to the heat-shock element and has homology to HSFs of other species. Mouse HSF2 was expressed in all three embryonic layers at embryonic day 7.5 and the head fold was strongly stained at embryonic day 8.5. In adults, expression is detected in spermatocytes and spermatogonia, but not in elongated spermatids, spermatozoa, or Sertoli cells. It mediates Hsp70i bookmarking, binding to this promoter in mitotic cells, recruits protein phosphatase-2A, and interacts with the CAPG subunit of the condensin enzyme to promote efficient dephosphorylation and inactivation of condensin complexes in the vicinity, thereby preventing compaction at this site. And HSF2-null mice were born at the expected mendelian ratio but had brain abnormalities and meiotic and gametogenic defects in both genders.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the middle region of human HSF2 (KQERDGPVEFQHPYFKQGQDD) was used as the immunogen for this HSF2 antibody.
Limitation:
This HSF2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q03933