HSP10 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30887
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30887-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the HSP10 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of HSP10 antibody and rat samples 1: thymus; 2: brain; 3: ovary; 4: testis; and human samples 5: A431; 6: A549; 7: MCF-7; 8: MM231; 9: HeLa cell lysate
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IHC-P: HSP10 antibody testing of human intestinal cancer tissue
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ICC testing of HSP10 antibody and A549 cells
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IHC-P: HSP10 antibody testing of mouse intestine tissue
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IHC staininging of FFPE rat intestinal tissue with HSP10 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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Immunofluorescent staining of FFPE human intestinal cancer with HSP10 antibody (red) and DAPI nuclear counterstain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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Immunofluorescent staining of FFPE human intestinal cancer with HSP10 antibody (red) and DAPI nuclear counterstain (blue). HIER: boil tissue sections in pH8 EDTA for 10-20 min followed by cooling at RT for 20 min.
8 / 12
IHC staining of FFPE human lung cancer with HSP10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Immunofluorescent staining of FFPE human HepG2 cells with HSP10 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human HeLa cells with HSP10 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
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Western blot testing of 1) human A431, 2) human A549, 3) rat heart and 4) mouse heart lysate with HSP10 antibody. Expected molecular weight: ~10 kDa.
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Flow cytometry testing of human HepG2 cells with HSP10 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HSP10 antibody.
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Western blot testing of HSP10 antibody and rat samples 1: thymus; 2: brain; 3: ovary; 4: testis; and human samples 5: A431; 6: A549; 7: MCF-7; 8: MM231; 9: HeLa cell lysate
IHC-P: HSP10 antibody testing of human intestinal cancer tissue
ICC testing of HSP10 antibody and A549 cells
IHC-P: HSP10 antibody testing of mouse intestine tissue
IHC staininging of FFPE rat intestinal tissue with HSP10 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Immunofluorescent staining of FFPE human intestinal cancer with HSP10 antibody (red) and DAPI nuclear counterstain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Immunofluorescent staining of FFPE human intestinal cancer with HSP10 antibody (red) and DAPI nuclear counterstain (blue). HIER: boil tissue sections in pH8 EDTA for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human lung cancer with HSP10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human HepG2 cells with HSP10 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human HeLa cells with HSP10 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human A431, 2) human A549, 3) rat heart and 4) mouse heart lysate with HSP10 antibody. Expected molecular weight: ~10 kDa.
Flow cytometry testing of human HepG2 cells with HSP10 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HSP10 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml, Immunocytochemistry: 0.5-1ug/ml,Immunofluorescence: 3-6ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the HSP10 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Heat shock 10kDa protein 1, also called CPN10, GROES, and HSP10, is a protein that in humans is encoded by the HSPE1 gene. It is a heptameric ring of identical 10.4-kD subunits that binds to each end of GroEL to form a symmetric, functional heterodimer. The transcriptional activity of the promoter fragment in the HSP60 direction is approximately twice that in the HSP10 direction under normal growth conditions; upon heat shock, promoter activity in either direction increased by a factor of approximately 12. Mutational drifts performed in vitro with 4 different enzymes indicated the HSP10 overexpression doubled the number of accumulating mutations, and promoted the folding of enzyme variants carrying mutations in the protein core and/or mutations with higher destabilizing effects.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human Heat shock protein 10 (VVLDDKDYFLFRDGDILGKYVD) was used as the immunogen for this HSP10 antibody.
Limitation:
This HSP10 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P61604