HSP105 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30688
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30688-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Hsp105 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of HSP105 antibody and Lane 1: rat ovary ; 2: A549 cell lysate; 3: U87 cell lysate; 4: HeLa cell lysate
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IHC-P: HSP105 antibody testing of human breast cancer tissue
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IHC-P: HSP105 antibody testing of rat intestine tissue
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IHC-F testing of rat intestine tissue
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Western blot testing of HSP105 antibody and Lane 1: rat ovary ; 2: A549 cell lysate; 3: U87 cell lysate; 4: HeLa cell lysate
IHC-P: HSP105 antibody testing of human breast cancer tissue
IHC-P: HSP105 antibody testing of rat intestine tissue
IHC-F testing of rat intestine tissue

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the HSP105 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Heat shock protein 105, also called HSPH1 or HSP110, is a protein that in humans is encoded by the HSPH1 gene. Immunohistochemical analysis localizes HSP105 mainly in the cytoplasm. Database analysis indicates that both HSP105 isoforms are highly conserved during evolution. By analysis of radiation hybrids and human/rodent hybrid cell lines, the HSPH1 gene is mapped to chromosome 13. Both HSP105alpha and HSP105beta are upregulated in HeLa cells exposed to heat shock. HSP105alpha, but not HSP105beta, is also upregulate in response to other cell stresses. Following heat shock, HSP105 relocalizes from a cytoplasmic to perinuclear position. Additionally, it may constitute a major determinant for both prognosis and treatment response in colorectal cancer.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 713-733 (EVMEWMNNVMNAQAKKSLDQD-human) were used as the immunogen for this HSP105 antibody.
Limitation:
This HSP105 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q92598