HSP25 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30041
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30041-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: IAP-9, aka SJ-25
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rabbit
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the HSP25 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Documents

Further Information

Application Details :
Western blot: 0.5-2ug/ml,IHC (Paraffin): 1-2ug/ml (1),Immunohistochemistry (Frozen): 1-2ug/ml--formalin or acetone fixed tissues,Immunocytochemistry: Suitable
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the HSP25 antibody may be required due to differences in protocols and secondary/substrate sensitivity. 1. Boil sections in 10mM Citrate buffer, pH6, to improve staining.
Description:
The heat-shock proteins(HSPs) belong to a larger group of polypeptides, the stress proteins, that are induced in various combinations in response to environmental challenges and developmental transitions. Heat-shock 27-kD protein1also knows as HSPB. Synthesis of the small(27-kD) HSP has been shown to be correlated with the acquisition of thermotolerance. HSP27 gene is mapped to 7q11.23. Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin.
Format :
Ascites
Formulation :
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Gene ID #:
3315
Immunogen:
Partially purified inhibitor of actin polymerization (IAP) protein from turkey gizzard smooth muscle was used as the immunogen for this HSP25 antibody.
Limitation:
This HSP25 antibody is available for research use only.
Purity:
Ascites
Species Reactivity :
Human, rat, mouse, rabbit