HSP27 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31650
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31650-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the HSP27 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of HSP27 antibody and Lane 1: rat NRK; 2: human HeLa; 3: (h) A549; 4: (h) COLO320; 5: (h) HEPG2 lysate. Expected size 23~27KD
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Western blot testing of HSP27 antibody and recombinant human protein (0.5ng)
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IHC-P: HSP27 antibody testing of human intestine cancer tissue
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IHC-P: HSP27 antibody testing of human breast cancer tissue
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IHC-F testing of HSP27 antibody and human placenta tissue
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Western blot testing of HSP27 antibody and Lane 1: rat NRK; 2: human HeLa; 3: (h) A549; 4: (h) COLO320; 5: (h) HEPG2 lysate. Expected size 23~27KD
Western blot testing of HSP27 antibody and recombinant human protein (0.5ng)
IHC-P: HSP27 antibody testing of human intestine cancer tissue
IHC-P: HSP27 antibody testing of human breast cancer tissue
IHC-F testing of HSP27 antibody and human placenta tissue

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the HSP27 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
HSPB1 (Heat shock 27kDa protein 1), also known as HSP27, is a protein that in humans is encoded by the HSPB1 gene. The protein encoded by this gene is induced by environmental stress and developmental changes. HSP27 is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
3315
Immunogen:
Human partial recombinant protein (AA 1-205) was used as the immunogen for this HSP27 antibody.
Limitation:
This HSP27 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human